Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays
نویسندگان
چکیده
منابع مشابه
Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays
The enzyme-linked immunospot (ELISPOT) assay has advanced into a useful and widely applicable tool for the evaluation of T-cell responses in both humans and animal models of diseases and/or vaccine candidates. Using synthetic peptides (either individually or as overlapping peptide mixtures) or whole antigens, total lymphocyte or isolated T-cell subset responses can be assessed either after shor...
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Measurements of serum-neutralizing antibody and anti-rotavirus immunoglobulin A (IgA) are the current standard for assessing immune responses following rotavirus vaccination. However, there is ongoing debate as to whether antibody titers correlate with protection against rotavirus gastroenteritis. Children recovering from rotavirus gastroenteritis have increased gamma interferon release from cu...
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Enzyme-linked immunospot (ELISPOT) analysis is a sensitive technique for the detection and quantification of antigen-specific T cells (5,7) and also for the detection of various cytokines released by immune cells (2). It is used extensively to monitor peptide-specific T cell responses in vaccination protocols (3), melanoma patients (6), and adoptive cellular immunotherapy (9) because it can det...
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Lymphocytes are crucial in the immune defence against Mycobacterium tuberculosis (MTB) infection. The aim of the present study was to ascertain whether or not MTB-specific lymphocytes are selectively compartmentalised in the lungs of patients with minimal active pulmonary tuberculosis (PTB). Patients with smear-negative MTB-culture-confirmed PTB were prospectively recruited. Differential cell c...
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PURPOSE Define an immunologic response using the tetramer and enzyme-linked immunospot (ELISPOT) assays. EXPERIMENTAL DESIGN Ten healthy subjects and 21 patients with melanoma (all HLA-A*0201) donated a total of 121 blood samples to determine the lower limit of detection (LLD), analytic coefficient of variation (aCV), and physiologic CV (pCV) of the tetramer and ELISPOT assays. The mean, SD, ...
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ژورنال
عنوان ژورنال: Clinical and Developmental Immunology
سال: 2013
ISSN: 1740-2522,1740-2530
DOI: 10.1155/2013/637649